6FEY
Crystal structure of Drosophila neural ectodermal development factor Imp-L2 with Drosophila DILP5 insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.91500 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 150.834, 150.834, 125.321 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 - 3.480 |
| R-factor | 0.26548 |
| Rwork | 0.261 |
| R-free | 0.34456 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cbp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.734 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 3.670 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmerge | 0.165 | 1.170 |
| Rpim | 0.089 | 0.630 |
| Number of reflections | 17857 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | Cp 10 mg/ml, 8-10% w/v PEG 4k or 6K, 20 mM MgCl2, 0.1 M HEPES pH 6.8-7.5 |
