6FCV
Structure of the human DDB1-CSA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 140.790, 140.790, 249.331 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.670 - 2.920 |
| R-factor | 0.19557 |
| Rwork | 0.193 |
| R-free | 0.24528 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a11 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.039 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.170 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.059 | 0.430 |
| Number of reflections | 55130 | 8564 |
| <I/σ(I)> | 6.8 | 1.2 |
| Completeness [%] | 89.5 | 92.75 |
| Redundancy | 3.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium citrate, 24% PEG 3350, 0.1 M bis-tris propane pH 8.0, 3% glycerol |
