6FCP
Crystal structure of 14-3-3 sigma in complex with Shroom3 P1244L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.533, 112.185, 62.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.548 - 1.450 |
| R-factor | 0.132 |
| Rwork | 0.131 |
| R-free | 0.15840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lw1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.885 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.550 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmeas | 0.074 | 0.712 |
| Number of reflections | 51510 | 2541 |
| <I/σ(I)> | 21.1 | 4.1 |
| Completeness [%] | 99.6 | |
| Redundancy | 12.9 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.095 M HEPES pH7.3 0.19M CaCl2 26% v/v PEG400 5% v/v glycerol |
