6FCG
Crystal structure of an endo-laminarinase from Formosa Hel1_33_131
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.336, 149.143, 107.217 |
| Unit cell angles | 90.00, 103.35, 90.00 |
Refinement procedure
| Resolution | 104.320 - 2.600 |
| R-factor | 0.20629 |
| Rwork | 0.205 |
| R-free | 0.23881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wtp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.668 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.320 | 2.650 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 87396 | 4421 |
| <I/σ(I)> | 6.9 | 2.6 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.2 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.03M Magnesium Chloride 0.1M MOPS pH 7 9% PEG8000 15% Ethyleneglycol |
