6FC0
Crystal structure of the eIF4E-eIF4G complex from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.587, 75.169, 48.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.710 - 1.293 |
| R-factor | 0.1654 |
| Rwork | 0.163 |
| R-free | 0.20090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fbz chain A |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.941 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.710 | 1.360 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Number of reflections | 54870 | 7178 |
| <I/σ(I)> | 11.5 | 1.6 |
| Completeness [%] | 93.9 | 85.3 |
| Redundancy | 3.1 | 3 |
| CC(1/2) | 0.584 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 10% w/v PEG 20000 20% PEG 500 MME 0.03 M diethyleneglycol 0.03 M triethyleneglycol 0.03 M tetraethyleneglycol 0.03 M pentaethyleneglycol 0.1 M Bicine/Trizma pH 8.5 |
