6FBZ
Crystal structure of the eIF4E-eIF4G complex from Chaetomium thermophilum in the cap-bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 63.960, 75.354, 48.435 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.763 - 1.496 |
R-factor | 0.1718 |
Rwork | 0.170 |
R-free | 0.20580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tpw |
RMSD bond length | 0.022 |
RMSD bond angle | 1.669 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.350 | 1.520 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 38441 | 1742 |
<I/σ(I)> | 16 | 1.2 |
Completeness [%] | 99.6 | 91.6 |
Redundancy | 12.7 | 10.7 |
CC(1/2) | 0.416 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 10% w/v PEG 20000 20% PEG 500 MME 0.02 M 1,6-hexanediol 0.02 M 1-butanol 0.02 M (RS)-1,2-propanediol 0.02 M 2-propanol 0.02 M 1,4-butanediol 0.02 M 1,3-propanediol 0.1 M Bicine/Trizma pH 8.5 0.687 mM m7GpppG |