6FBR

Crystal Structure of the Human Retinoid X Receptor DNA-Binding Domain Bound to the Human MEp DR1 Response Element, pH 4.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.2821
Spacegroup name	P 31 2 1
Unit cell lengths	55.659, 55.659, 166.131
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.290 - 2.100
R-factor	0.181
Rwork	0.178
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fbq
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.290	46.290	2.120
High resolution limit [Å]	2.000	5.960	2.000
Rmerge	0.113	0.034	3.280
Rmeas	0.127	0.039	3.732
Total number of observations	87918
Number of reflections	19015	896	1948
<I/σ(I)>	8.79	34.41	0.39
Completeness [%]	90.2	96.3	58.9
Redundancy	4.624	4.064	4.18
CC(1/2)	0.998	0.999	0.152

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	40% PEG 300, 0.1M sodium phosphate citrate pH 4.2