6FBE

KlenTaq DNA polymerase processing a modified primer - bearing the modification upstream at the third primer nucleotide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-02
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999990
Spacegroup name	P 31 2 1
Unit cell lengths	108.480, 108.480, 90.309
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.973 - 1.589
R-factor	0.1766
Rwork	0.175
R-free	0.20320
RMSD bond length	0.006
RMSD bond angle	0.704
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12rc1_2815: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.973	1.597
High resolution limit [Å]	1.589	1.589
Rmeas	0.108	1.404
Number of reflections	159958	25710
<I/σ(I)>	8.39	0.9
Completeness [%]	99.8	99.3
Redundancy	4.79	4.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	20 % PEG 4000, 0.1 M HEPES, 20 mM manganese(II) chloride, 0.1 M sodium acetate