6FB2
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.89 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.889, 67.362, 90.867 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 41.986 - 2.950 |
| R-factor | 0.1916 |
| Rwork | 0.187 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9z |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.803 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.570 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 11150 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol |
