6FAX

Complex of Human CD40 Ectodomain with Lob 7.4 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976254
Spacegroup name	P 31 2 1
Unit cell lengths	158.753, 158.753, 93.622
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	137.480 - 2.990
R-factor	0.18707
Rwork	0.185
R-free	0.23121
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u6a
RMSD bond length	0.018
RMSD bond angle	2.134
Data reduction software	xia2 (0.4.0.338-g7e19e23-dials-1.2)
Data scaling software	Aimless (0.5.27)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.000	3.180
High resolution limit [Å]	2.990	2.990
Rmerge	0.161
Number of reflections	27589
<I/σ(I)>	13.7	2.4
Completeness [%]	99.9	99.3
Redundancy	13.7	6.2
CC(1/2)	0.998	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	NA
2	VAPOR DIFFUSION, HANGING DROP		293	NA