6FAD

SR protein kinase 1 (SRPK1) in complex with the RGG-box of HSV1 ICP27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 32 1 2
Unit cell lengths	99.980, 99.980, 426.049
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	80.210 - 2.801
R-factor	0.2009
Rwork	0.198
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wak
RMSD bond length	0.008
RMSD bond angle	0.966
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.210	2.901
High resolution limit [Å]	2.801	2.801
Rmerge	0.095	0.753
Rmeas	0.100	0.800
Rpim	0.031	0.268
Number of reflections	60695	5993
<I/σ(I)>	17.5	2.54
Completeness [%]	100.0	99.97
Redundancy	10.1	8.9
CC(1/2)	0.994	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.09 M (Sodium nitrate, 0.3 Sodium phosphate, 0.3 M ammonium sulphate), 0.1 M (Sodium HEPES, MOPS) buffer system, 50% v/v GOL_P4K mix [Morpheus HT96 C7, Molecular Dimensions]