6F89
Structure of H234A/Y235A P.abyssi Sua5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.02 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.440, 125.270, 47.940 |
Unit cell angles | 90.00, 91.84, 90.00 |
Refinement procedure
Resolution | 47.920 - 2.810 |
R-factor | 0.1644 |
Rwork | 0.162 |
R-free | 0.20870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2eqa |
RMSD bond length | 0.014 |
RMSD bond angle | 1.735 |
Data reduction software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.920 | 2.980 |
High resolution limit [Å] | 2.810 | 2.811 |
Rmeas | 0.074 | |
Number of reflections | 24980 | |
<I/σ(I)> | 9.22 | |
Completeness [%] | 97.8 | |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | ammonium sulfate |