6F5F

Structure of ARTD2/PARP2 WGR domain bound to double strand DNA with 5 nucleotide overhang and 5'phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	68.150, 87.150, 185.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.860 - 2.980
R-factor	0.23056
Rwork	0.228
R-free	0.27530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 6F1K
RMSD bond length	0.011
RMSD bond angle	1.778
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.980	2.980
Rmerge	0.069
Rmeas	0.075
Number of reflections	23275
<I/σ(I)>	15.29
Completeness [%]	99.8
Redundancy	6.38
CC(1/2)	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Na-formate 20 % PEG 3350 20 % glycerol