6F4S
Human JMJD5 (N308C) in complex with Mn(II), 2OG and RCCD1 (139-143) (complex-4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.321, 65.607, 77.691 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.426 - 1.461 |
R-factor | 0.1375 |
Rwork | 0.137 |
R-free | 0.15200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f4r |
RMSD bond length | 0.008 |
RMSD bond angle | 0.962 |
Data reduction software | HKL-2000 (706E) |
Data scaling software | HKL-2000 (706E) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.430 | 1.490 |
High resolution limit [Å] | 1.460 | 1.460 |
Rmerge | 0.113 | 1.300 |
Rpim | 0.050 | 0.602 |
Number of reflections | 44375 | 2928 |
<I/σ(I)> | 17 | 2 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7 | 7 |
CC(1/2) | 0.997 | 0.602 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Bis-Tris pH 6.5, 29 % PEG3350, 0.002 M MnCl2, 300 nl sitting drops (sample:well, 1:2 ratio) |