6F4P
Human JMJD5 in complex with MN, NOG and RPS6 (129-144) (complex-1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.283, 64.925, 78.037 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.460 - 1.450 |
R-factor | 0.1473 |
Rwork | 0.146 |
R-free | 0.16820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f4o |
RMSD bond length | 0.009 |
RMSD bond angle | 1.005 |
Data reduction software | HKL-2000 (706E) |
Data scaling software | HKL-2000 (706E) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.460 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.090 | 1.200 |
Rpim | 0.035 | 0.497 |
Number of reflections | 44727 | 4347 |
<I/σ(I)> | 19 | 1.8 |
Completeness [%] | 98.8 | 97.7 |
Redundancy | 8.8 | 8.4 |
CC(1/2) | 0.997 | 0.634 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.1M Bis-Tris pH 6.0, 26.4 % PEG3350, 0.002 M MnCl2, 300 nl sitting drops (sample:well, 1:2 ratio) |