6EZT

Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase D437A inactive mutant from Vibrio harveyi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97889
Spacegroup name	P 1 21 1
Unit cell lengths	89.420, 129.240, 98.360
Unit cell angles	90.00, 112.20, 90.00

Refinement procedure

Resolution	91.090 - 2.600
R-factor	0.197
Rwork	0.195
R-free	0.23650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ezr
RMSD bond length	0.007
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	91.090	91.090	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.125	0.075	0.775
Rmeas	0.136	0.081	0.835
Number of reflections	63617	735	4675
<I/σ(I)>	9.7	20.27	2.18
Completeness [%]	99.8	98	99.7
Redundancy	6.861	6.796	7.102
CC(1/2)	0.993	0.993	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.5	293	0.1 M sodium acetate pH 4.6, 1.4 M sodium malonate