6EZB

Crystal Structure of human tRNA-dihydrouridine(20) synthase catalytic domain Q305K mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980105
Spacegroup name	I 2 2 2
Unit cell lengths	72.080, 84.110, 144.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.610 - 2.250
R-factor	0.183
Rwork	0.181
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xp7
RMSD bond length	0.010
RMSD bond angle	1.130
Data reduction software	XDS (20180409)
Data scaling software	STARANISO (1.10.15)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.610	2.450
High resolution limit [Å]	2.250	2.250
Rmerge	0.137	1.609
Rmeas		1.714
Number of reflections	16301
<I/σ(I)>	12.6	1.4
Completeness [%]	93.4	93.53
Redundancy	9.2	8.7
CC(1/2)	0.998	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	2.2 M ammonium sulfate