6EYO

Structure of extended IgE-Fc in complex with two anti-IgE Fabs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	119.483, 119.601, 132.994
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.930 - 3.700
R-factor	0.2496
Rwork	0.247
R-free	0.28820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wqr
RMSD bond length	0.002
RMSD bond angle	0.510
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.930	4.050
High resolution limit [Å]	3.700	3.700
Rmerge	0.224	0.691
Rpim	0.094	0.284
Number of reflections	20732	4698
<I/σ(I)>	6.8	2.8
Completeness [%]	98.8	95.3
Redundancy	6.6	6.8
CC(1/2)	0.989	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.1M Tris-HCl pH8.0 and 45% (v/v) pentaerythritol propoxylate (5/4PO/OH)