6EY6

C-terminal part (residues 315-516) of PorM with the llama nanobody nb130

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97857
Spacegroup name	P 1
Unit cell lengths	55.245, 77.179, 156.302
Unit cell angles	90.24, 91.76, 97.16

Refinement procedure

Resolution	38.350 - 2.100
R-factor	0.1823
Rwork	0.181
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ey5 5fwo
RMSD bond length	0.010
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.210
High resolution limit [Å]	2.100	2.100
Rmeas	0.065	0.764
Rpim		0.433
Number of reflections	145898	221179
<I/σ(I)>	12.5	2
Completeness [%]	97.6	96.8
Redundancy	2.9	2.9
CC(1/2)		0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M ammonium citrate tribasic pH 7.0 20%(w/v) PEG3350