6EWX
Structure of Pragmin pseudo-kinase reveals a dimerization mechanism to regulate protein tyrosine phosphorylation and nuclear transcription
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97294 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.430, 58.920, 110.640 |
| Unit cell angles | 90.00, 118.48, 90.00 |
Refinement procedure
| Resolution | 54.999 - 2.771 |
| R-factor | 0.1905 |
| Rwork | 0.188 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.151 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.8.4) |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.870 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.052 | 0.614 |
| Number of reflections | 25025 | |
| <I/σ(I)> | 12.2 | 2.1 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 4.1 | 4.2 |
| CC(1/2) | 0.999 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | ammonium sulphate 1M, lithium citrate 0.2 M pH 5.0 and 12.5 mM EDTA |
