6EVF
Structure of E285D S. cerevisiae Fdc1 with prFMN in the hydroxylated form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | .979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.100, 96.820, 116.780 |
| Unit cell angles | 90.00, 96.61, 90.00 |
Refinement procedure
| Resolution | 34.350 - 2.060 |
| R-factor | 0.16965 |
| Rwork | 0.167 |
| R-free | 0.21273 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zac |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.821 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.350 | 3.134 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmeas | 1.071 | |
| Number of reflections | 154138 | 15457 |
| <I/σ(I)> | 8.35 | |
| Completeness [%] | 99.0 | |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M sodium cacodylate pH 6.5, 0.25M Calcium Acetate, 15% PEG 4k |
