6EUO
Crystal structure of APO Fe(II)/alpha-ketoglutarate dependent dioxygenase KDO5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.280, 98.930, 166.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.200 - 2.300 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.200 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 67467 | 4925 |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.2 | 5 |
| CC(1/2) | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 23% PEG 3350, 200mM Imidazole malate pH7, 150mM Li2SO4 |
