6ES8
HIV capsid hexamer with IP6 ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2017-01-03 |
| Detector | OXFORD RUBY CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 6 |
| Unit cell lengths | 91.299, 91.299, 57.201 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.390 - 1.900 |
| R-factor | 0.18763 |
| Rwork | 0.186 |
| R-free | 0.22372 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hgp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.931 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.084 | 0.878 |
| Number of reflections | 21569 | |
| <I/σ(I)> | 10.8 | 1.6 |
| Completeness [%] | 91.5 | 94.1 |
| Redundancy | 4.6 | 4.4 |
| CC(1/2) | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 277 | PEG4K |
