6ES3

Structure of CDX2-DNA(TCG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9724
Spacegroup name	C 1 2 1
Unit cell lengths	127.950, 46.490, 68.890
Unit cell angles	90.00, 113.27, 90.00

Refinement procedure

Resolution	43.230 - 2.570
R-factor	0.2221
Rwork	0.216
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lty
RMSD bond length	0.017
RMSD bond angle	2.053
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.230	43.230	2.240
High resolution limit [Å]	2.180	9.760	2.180
Rmerge	0.185	0.084	2.991
Rmeas	0.191	0.087	3.273
Number of reflections	19575	243	1388
<I/σ(I)>	8.47	28.25	0.43
Completeness [%]	99.6	98.8	97.8
Redundancy	16.093	17.424	6.019
CC(1/2)	0.998	0.998	0.107

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	19% PME 5000, 0.15 M potassium chloride, 0.1 M magnesium chloride, 8% PEG 400, 0.05M TRIS-buffer pH 8.0