6EQZ

A MamC-MIC insertion in MBP scaffold at position K170

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	110.682, 113.813, 115.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.696 - 2.293
R-factor	0.2082
Rwork	0.205
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e7u
RMSD bond length	0.009
RMSD bond angle	1.193
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.700	2.350
High resolution limit [Å]	2.290	2.290
Rmerge	0.252	2.133
Number of reflections	65839
<I/σ(I)>	5.5
Completeness [%]	99.6
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	1,86 M Tris-Ammonium citrate