6EPK

CRYSTAL STRUCTURE OF THE PRECURSOR MEMBRANE PROTEIN-ENVELOPE PROTEIN HETERODIMER FROM THE YELLOW FEVER VIRUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.
Spacegroup name	P 41
Unit cell lengths	99.880, 99.880, 238.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.717 - 2.700
R-factor	0.1628
Rwork	0.162
R-free	0.18630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c5x
RMSD bond length	0.003
RMSD bond angle	0.672
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AMoRE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.090	1.123
Rmeas	0.111	1.394
Rpim	0.063	0.814
Number of reflections	63786	4469
<I/σ(I)>	17	1.2
Completeness [%]	99.9	100
Redundancy	5.4	5.1
CC(1/2)	0.997	0.519

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	1.2 M Li2SO4, 100mM TrisHCl pH8