6ENW
X-ray structure of Auoxo4-encapsulated horse spleen apoferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-14 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.878, 180.878, 180.878 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.430 - 2.600 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5erk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.430 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.187 | 0.414 |
| Rpim | 0.145 | 0.404 |
| Number of reflections | 8209 | |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 99.0 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.6-0.8 M (NH4)2SO4, 0.1 M Tris pH 7.4-7.7, 50-60 mM CdSO4 |
