6ENP
Atomic resolution structure of human RNase 6 in the presence of phosphate anions in P21 space group.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.734, 38.557, 98.965 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.927 - 1.042 |
| R-factor | 0.1227 |
| Rwork | 0.121 |
| R-free | 0.14490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4x09 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.424 |
| Data reduction software | XDS (20151001) |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHENIX (1.11.1_2575) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.930 | 1.079 |
| High resolution limit [Å] | 1.042 | 1.042 |
| Rmerge | 0.032 | 0.506 |
| Rmeas | 0.038 | 0.019 |
| Rpim | 0.019 | 0.306 |
| Number of reflections | 95760 | 4946 |
| <I/σ(I)> | 17.08 | 2.4 |
| Completeness [%] | 97.2 | 97.13 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.999 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1 M ammonium phosphate, 100 mM sodium acetate pH 4.5 |
