6EMX
X-ray structure of the N15'C mutant of GLIC in complex with bromoform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.790, 133.322, 158.767 |
| Unit cell angles | 90.00, 101.68, 90.00 |
Refinement procedure
| Resolution | 28.620 - 3.200 |
| R-factor | 0.247 |
| Rwork | 0.246 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 60179 |
| <I/σ(I)> | 8.7 |
| Completeness [%] | 99.0 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 12-14% PEGK 400mM NaSCN 100 mM NaAcetate pH 4 15% glycerol 3% DMSO |
