6EM7
Crystal Structure of the Protein-Kinase A catalytic subunit from Criteculus Griseus in complex with compounds RKp120 and ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.481, 80.009, 84.301 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.151 - 1.238 |
R-factor | 0.1394 |
Rwork | 0.139 |
R-free | 0.15650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.154 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.151 | 1.310 |
High resolution limit [Å] | 1.240 | 1.240 |
Number of reflections | 122237 | 19106 |
<I/σ(I)> | 19.02 | 2.83 |
Completeness [%] | 98.4 | 96.1 |
Redundancy | 4.42 | 4.3 |
CC(1/2) | 0.999 | 0.828 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 277.15 | 100 mM Mes-Bis-Tris, 55 mM lithium chloride, 10 mM magnesium chloride, 1 mM DTT, 0.1 mM sodium EDTA, 0.25 mM Mega 8, 0.7 mM peptidic ligand, 5 mM ADP, 20% v/v methanol/water in reservoir |