6EKO

Crystal structure of Type IIP restriction endonuclease PfoI with cognate DNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.96112,0.98010
Spacegroup name	P 21 21 21
Unit cell lengths	56.279, 91.663, 152.742
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.960 - 2.284
R-factor	0.2094
Rwork	0.205
R-free	0.24780
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.085
Data reduction software	XDS (VERSION Jun 17, 2015 BUILT=20150617)
Data scaling software	SCALA (CCP4 6.2: Scala version 3.3.20 : 23/06/11)
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.960	2.410
High resolution limit [Å]	2.280	2.280
Rmerge	0.198
Rmeas	0.216
Rpim	0.058	0.442
Number of reflections	36181	4683
<I/σ(I)>	11.6	1.8
Completeness [%]	98.5	89.8
Redundancy	13.5	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	Reservoir: 20% PEG8000, 0.1M TrisHCl pH8.5, LiCl 0.2M and glycerol 10%. Protein-DNA complex concentration 5.3 mg/ml