6EIU
Crystal structure of KDM5B in complex with KDOPZ29a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 143.380, 143.380, 153.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.384 - 1.880 |
| R-factor | 0.1969 |
| Rwork | 0.196 |
| R-free | 0.22090 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.866 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 124.170 | 1.880 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.101 | 2.858 |
| Number of reflections | 82282 | 6007 |
| <I/σ(I)> | 19.1 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20 | 20.8 |
| CC(1/2) | 1.000 | 0.604 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES pH 7.5, 0.8M Pottassium Phosphate-dibasic, 0.8M Sodium Phosphate monobasic |
