6EI8
Crystal structure of human tRNA-dihydrouridine (20) synthase dsRBD F359A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-29 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.110, 81.110, 56.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.810 - 2.250 |
| R-factor | 0.262 |
| Rwork | 0.259 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wft |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.810 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.233 | |
| Number of reflections | 10384 | 1020 |
| <I/σ(I)> | 9.34 | 1.01 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.2 | 11.6 |
| CC(1/2) | 0.999 | 0.746 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 2M ammonium sulfate 50 mM sodium cacodylate 15 mM magnesium acetate |
