6EHI
NucT from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.350, 71.370, 110.470 |
| Unit cell angles | 81.36, 74.30, 82.33 |
Refinement procedure
| Resolution | 47.410 - 1.580 |
| R-factor | 0.2035 |
| Rwork | 0.201 |
| R-free | 0.24630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ggk |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.065 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.410 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmeas | 0.070 | 0.595 |
| Number of reflections | 227845 | 35428 |
| <I/σ(I)> | 11.2 | 2.22 |
| Completeness [%] | 93.0 | 89.5 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.997 | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | PEG 4000 28% Ammonium sulfate Sodium acetate pH 6 |
