6EH3
Crystal Structure of the Protein-Kinase A catalytic subunit from Criteculus Griseus in complex with compounds RKp120 and RKp190
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.684, 73.526, 108.415 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.866 - 1.950 |
R-factor | 0.1753 |
Rwork | 0.173 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.009 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.866 | 2.070 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 34590 | 5401 |
<I/σ(I)> | 21.96 | 3.17 |
Completeness [%] | 98.7 | 97.1 |
Redundancy | 4.37 | 4.17 |
CC(1/2) | 0.999 | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 277.15 | 100 mM Mes-Bis-Tris, 75 mM lithium chloride, 1 mM DTT, 0.1 mM sodium EDTA, 0.25 mM Mega 8, 0.7 mM peptidic ligand, 5 mM RKp190, 19% v/v methanol/water in reservoir |