6EFD

Hsa Siglec and Unique domains in complex with the sialyl T antigen trisaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	46.645, 58.140, 75.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.168 - 1.850
R-factor	0.1968
Rwork	0.196
R-free	0.21760
RMSD bond length	0.015
RMSD bond angle	1.392
Refinement software	PHENIX ((1.14_3211))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.890
High resolution limit [Å]	1.850	1.850
Number of reflections	30549
<I/σ(I)>	31.7
Completeness [%]	98.8
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M SPG pH 10, 25% PEG 3350