6EF4
Crystal structure of mouse PP2A Aalpha P179R mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 122.523, 122.523, 160.963 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.790 - 3.400 |
| R-factor | 0.2196 |
| Rwork | 0.219 |
| R-free | 0.23530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iae |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.845 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 7.780 | 3.400 |
| Rmerge | 0.087 | 0.037 | |
| Rmeas | 0.094 | 0.041 | |
| Rpim | 0.039 | 0.017 | 0.456 |
| Total number of observations | 112094 | ||
| Number of reflections | 17427 | 1583 | 1396 |
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 99.7 | 98.3 | 98.7 |
| Redundancy | 6.4 | 5.9 | 6.3 |
| CC(1/2) | 0.999 | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Sodium malonate pH 7.0, 20% w/v Polyethylene glycol 3,350 |
