6EA9
Structure of VACV Poxin in post-reactive state with Gp[2'-5']Ap[3']
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-07-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 215.416, 57.147, 133.113 |
Unit cell angles | 90.00, 120.37, 90.00 |
Refinement procedure
Resolution | 38.281 - 2.100 |
R-factor | 0.2 |
Rwork | 0.199 |
R-free | 0.22390 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.885 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.281 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.035 | 0.681 |
Number of reflections | 81341 | 4327 |
<I/σ(I)> | 9.9 | 1.1 |
Completeness [%] | 99.2 | 97.2 |
Redundancy | 3.5 | 3.2 |
CC(1/2) | 0.999 | 0.847 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM NaOAc pH 4.8, 10-11% PEG-20,000 |