6E8T
Structure of the Mango-III (A10U) aptamer bound to TO1-Biotin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.105 |
| Spacegroup name | H 3 |
| Unit cell lengths | 91.945, 91.945, 161.691 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.970 - 2.900 |
| R-factor | 0.1988 |
| Rwork | 0.195 |
| R-free | 0.23650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e8s |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.370 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.970 | 3.030 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.038 | 0.910 |
| Number of reflections | 18895 | 1235 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 98.5 | 99 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 1.6 M Ammonium Citrate, 3.0% Glycerol, 4% Acetone |
