6E62

Crystal structure of malaria transmission-blocking antigen Pfs48/45 6C in complex with antibody 85RF45.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979490
Spacegroup name	P 21 21 21
Unit cell lengths	54.510, 74.450, 321.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.697 - 2.700
R-factor	0.2472
Rwork	0.246
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.629
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.700	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	37228	3756
<I/σ(I)>	14.4	1.7
Completeness [%]	100.0	100
Redundancy	13.1	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Bis-Tris, pH 5.5, 0.1 M ammonium acetate, 17% (w/v) PEG 10000