6E4L

The structure of the N-terminal domain of human clathrin heavy chain 1 (nTD) in complex with ES9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976300
Spacegroup name	P 21 21 21
Unit cell lengths	71.210, 74.340, 84.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.424 - 1.600
R-factor	0.1888
Rwork	0.188
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g55
RMSD bond length	0.008
RMSD bond angle	0.908
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.424	1.657
High resolution limit [Å]	1.600	1.600
Number of reflections	58329
<I/σ(I)>	17.53
Completeness [%]	97.8
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	20% PEG 3350, 150 MM POTASSIUM ACETATE, 0.1 M TRIS, pH 8.0