6E3T
Crystal Structure of the Heterodimeric HIF-2 Complex with Antagonist T1001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.032, 97.981, 77.427 |
| Unit cell angles | 90.00, 107.10, 90.00 |
Refinement procedure
| Resolution | 41.793 - 3.000 |
| R-factor | 0.2485 |
| Rwork | 0.245 |
| R-free | 0.30640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zp4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.586 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2467: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.010 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Rmerge | 0.075 | 0.714 |
| Number of reflections | 14016 | 1077 |
| <I/σ(I)> | 18.7 | 1.1 |
| Completeness [%] | 94.3 | 71.1 |
| Redundancy | 4.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2% TACSIMATE, PH 7.0, 6% PEG3350 |
