6E3I
Human Bfl-1 in complex with the Bfl-1-specific designed peptide srt.F4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.220, 42.947, 46.762 |
| Unit cell angles | 90.00, 114.73, 90.00 |
Refinement procedure
| Resolution | 39.255 - 1.481 |
| R-factor | 0.142 |
| Rwork | 0.139 |
| R-free | 0.17290 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 4.020 | 1.480 |
| Rmerge | 0.061 | 0.040 | 0.344 |
| Rmeas | 0.069 | 0.044 | 0.411 |
| Rpim | 0.030 | 0.019 | 0.218 |
| Number of reflections | 23986 | 1282 | 737 |
| <I/σ(I)> | 7.7 | ||
| Completeness [%] | 92.5 | 94.3 | 57.4 |
| Redundancy | 4.5 | 4.9 | 2.7 |
| CC(1/2) | 0.998 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.8 M ammonium sulfate, 0.1 M MES pH 7.0 |
