6E2B
Ubiquitin in complex with Pt(2-phenilpyridine)(PPh3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 140.381, 140.381, 62.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.260 - 1.450 |
| R-factor | 0.18234 |
| Rwork | 0.181 |
| R-free | 0.20735 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.359 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.260 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.160 | |
| Rmeas | 0.168 | |
| Rpim | 0.069 | 0.566 |
| Number of reflections | 110120 | 5428 |
| <I/σ(I)> | 13.8 | 1.58 |
| Completeness [%] | 99.0 | 36.7 |
| Redundancy | 12.9 | 10.8 |
| CC(1/2) | 0.476 | 0.476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 3M ammonium sulfate, 0.1M sodium citrate buffer, pH 5.5 |
