6DZH

HRAS G13D bound to GDP (H13GDP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-11-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	60.571, 71.410, 94.861
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.700 - 1.950
Rwork	0.163
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e6p
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.950	5.290	1.950
Rmerge	0.092	0.036	0.596
Rmeas	0.100	0.039	0.695
Rpim	0.037	0.014	0.345
Total number of observations	175531
Number of reflections	25256	1696	713
<I/σ(I)>	8.1
Completeness [%]	82.0	99.7	47.3
Redundancy	6.5	7.4	3.2
CC(1/2)		0.999	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	5.9% stabilization buffer (20 mM HEPES, 50 mM NaCl, 20 mM MgCl2, pH 7.5) 188.2 mM Calcium Acetate 18.8% PEG 3350 Crystals were grown in 2uL by 2uL drops of mother liquor to protein (7.7mg/mL) Cryoprotectant for crystal diffraction: 70% mother liquor and 30% glycerol