6DWO
Crystal structure of alpha-1-2-mannosidase from Enterococcus faecalis V583
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 163.350, 168.990, 258.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.180 - 2.150 |
R-factor | 0.15698 |
Rwork | 0.155 |
R-free | 0.18893 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5swi |
RMSD bond length | 0.008 |
RMSD bond angle | 1.511 |
Data reduction software | XDS (Jun 1, 2017 BUILT=20170601) |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.210 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.096 | 0.678 |
Rmeas | 0.111 | 0.703 |
Number of reflections | 190871 | 14034 |
<I/σ(I)> | 11.12 | 1.93 |
Completeness [%] | 99.0 | 99.2 |
Redundancy | 4 | 4 |
CC(1/2) | 0.997 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.15 M sodium acetate, pH 4.5, 0.8 M sodium phosphate monobasic, 1.2 M potassium phosphate dibasic |