6DVR

Crystal structure of human CARM1 with (R)-SKI-72

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97890
Spacegroup name	I 2 2 2
Unit cell lengths	74.366, 98.579, 207.375
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.560 - 1.540
R-factor	0.1949
Rwork	0.195
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ikp
RMSD bond length	0.006
RMSD bond angle	1.177
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.570
High resolution limit [Å]	1.540	4.180	1.540
Rmerge	0.049	0.028	0.977
Rmeas	0.052	0.029	1.028
Rpim	0.016	0.009	0.316
Number of reflections	112857	5945	5573
<I/σ(I)>	7.3
Completeness [%]	100.0	99.8	100
Redundancy	10.7	10.5	10.4
CC(1/2)		0.999	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	25% PEG 3350, 0.2 M NH4SO4, 0.1 M HEPES pH7.5