6DUY

Crystal structure of the alpha-N-catenin actin-binding domain H1 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-15
Detector	RAYONIX MX-300
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	75.094, 82.845, 105.309
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.440 - 2.810
R-factor	0.2042
Rwork	0.199
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k1o
RMSD bond length	0.009
RMSD bond angle	0.926
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.440	2.910
High resolution limit [Å]	2.800	2.800
Rmerge	0.072	0.687
Number of reflections	16455
<I/σ(I)>	21.7	2.8
Completeness [%]	99.9	100
Redundancy	7.3	7.4
CC(1/2)	0.970	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	100 mM sodium acetate/acetic acid, pH 4.5, 0.8 M sodium phosphate monobasic, 1.2 M potassium phosphate dibasic