6DUQ
Structure of a Rho-NusG KOW domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 1 |
| Unit cell lengths | 126.350, 126.320, 166.820 |
| Unit cell angles | 89.94, 89.90, 60.01 |
Refinement procedure
| Resolution | 39.030 - 3.700 |
| R-factor | 0.288 |
| Rwork | 0.287 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.772 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.790 |
| High resolution limit [Å] | 3.680 | 3.680 |
| Number of reflections | 94588 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 97.8 | 97.6 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 95 mM potassium glutamate, 50 mM Tris-HCl pH 8.0, 1 mM spermine, 0.5% PEG 8K |
