6DTZ

Crystal structure of eukaryotic DNA primase large subunit iron-sulfur cluster domain, Y397F mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-24
Detector	Bruker Platinum 135
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	40.806, 50.810, 90.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.120 - 1.360
R-factor	0.157
Rwork	0.155
R-free	0.17900
Structure solution method	SAD
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	SHELXDE
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.120	1.410
High resolution limit [Å]	1.360	1.360
Rmerge	0.046	0.328
Number of reflections	40483	3945
<I/σ(I)>	12.72	2.21
Completeness [%]	98.7	97.87
Redundancy	1.9	1.8
CC(1/2)		0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	100 mM TRIS, 40% MPD